Title:
【発明の名称】ヒトTNF結合タンパク質▲I▼(TNF-BP▲I▼)に対するモノクローナル抗体
Document Type and Number:
Japanese Patent JP3425146
Kind Code:
B2
Abstract:
Monoclonal antibodies (MAb) against TNF-BPI (tumour necrosis factor-binding protein I), designated tbp-1 and tbp-2 and secreted by the hybridomas TBP-1 and -2, and their active fragments are new. Also new are (1) hybridomas TBP-1 and-2 (ECACC 91060555 and 91060556, respectively) and (2) sandwich immunoassay kits for determn. of TNF-BPI in a sample. More specifically, TNF-BPI is detected by reaction with at least one MAb to form a binary or ternary complex. In partic. a ternary complex is formed esp. where the 1st. antibody is tbp-1 and the 2nd. is tbp-2. USE/ADVANTAGE - MAb provide an efficient and sensitive method for detecting TNF-BPI in body fluids (serum, plasma or urine) and cell culture supernatants. The method is used to diagnose (and monitor) diseases associated with activation of the TNF-receptor system, specifically septic shock but also bacterial infections, graft vs. host disease, cerebral malaria and cachexia. TNF-BPI can be determined at 200 ng/l with reproducibility better than 10% and the presence of TNF in the sample does not affect the assay, even at pathologically elevated levels. MAb can also be used for affinity chromatographic purificn. of TNF-BPI
Inventors:
Adolf Gunther
Application Number:
JP51105292A
Publication Date:
July 07, 2003
Filing Date:
June 13, 1992
Export Citation:
Assignee:
Boehringer Ingelheim International Gesellschaft Mitt Beschlenktel Haftung
International Classes:
A61K39/395; A61P43/00; C07K16/18; C07K16/28; C12N5/10; C12N15/02; C12P21/08; G01N33/53; G01N33/577; A61K38/00; C12R1/91; (IPC1-7): C07K16/18; A61K39/395; A61P43/00; C12N5/10; C12N15/02; C12P21/08; G01N33/53; G01N33/577
Domestic Patent References:
| JP2154695A | ||||
| JP61213670A | ||||
| JP62192662A |
Other References:
【文献】欧州特許出願公開412486(EP,A1)
【文献】Journal of Immunological Methods,Vol.143,(1991)p.
【文献】Journal of Immunological Methods,Vol.143,(1991)p.
Attorney, Agent or Firm:
Minoru Nakamura (7 outside)