Title:
【発明の名称】新規な抗原提示細胞、その調製方法、および細胞性ワクチンとしてのその用途
Document Type and Number:
Japanese Patent JP3428025
Kind Code:
B2
Abstract:
The invention relates to macrophages characterized in that they have the following properties: they present on their surface : antigen CD14 with a mean intensity of about 20 to about 200, antigen CD64 with a mean intensity of about 20 to about 200, they are substantially devoid of the surface antigens CD1a and CD1c, the presence and mean intensities respectively of CD14, CD64 and the absence of CD1a and CD1c being for instance determined by immunofluorescence staining and flow cytometry analysis, they present a phagocytosis property such as determined by the following test: said phagocytosis capacity being evaluated by an uptake of formalin fixed yeast, for example by culturing macrophages for 2 hours, adding yeast in 1/10 macrophages/yeast ratio and incubating at 37 DEG C, 5% CO2 atmosphere for 2-3 hours fixing by the May-Gr}nwald-Giems a (MGG) staining, and the percentage of phagocytic macrophages being quantified for instance by microscopic analysis, they have the property of stimulating the proliferation of allogenic lymphocytes such as determined by the following test : allogenic primary mixed lymphocytes reaction (MLR) was carried out in 96-well microtiter plates by adding different numbers (2x10<3> to 2x10<5> in 100 mu l medium/well) of macrophages to 2x10<5> in 100 mu l medium/well of allogenic T cells purified from buffy coats and after 5 days incubation at 37 DEG C, cell proliferation was assessed by a colorimetric method, such as the hydrolysis of tetrazolium salt WST-1 (Boehringer Mannheim, Germany), (slightly red) to Formozan (dark red).
Inventors:
Chokuri, Mohammed
Bartolan, Jack
Rohm-leumont, jean-lou
Bartolan, Jack
Rohm-leumont, jean-lou
Application Number:
JP54158397A
Publication Date:
July 22, 2003
Filing Date:
May 15, 1997
Export Citation:
Assignee:
I.D.M.IMMUNO-DESIGNED MOLECULES
International Classes:
G01N33/53; A61K31/00; A61K31/415; A61K31/4164; A61K31/417; A61K35/14; A61K35/15; A61K35/17; A61K38/00; A61K39/00; A61K39/02; A61K45/00; A61P37/00; A61P37/04; C07K14/705; C07K16/46; C12N5/02; C12N5/0786; C12N5/10; C12N15/09; C12P21/02; A61K35/12; (IPC1-7): C12N5/06; A61K31/415; A61K35/14; A61K38/00; A61K39/00; A61K39/02; A61K45/00; C07K14/705; G01N33/53
Domestic Patent References:
| JP6502410A |
Other References:
【文献】国際公開94/26875(WO,A1)
Attorney, Agent or Firm:
Hajime Tsukuni (1 person outside)