Title:
微細加工装置を用いたポリヌクレオチド増幅分析
Document Type and Number:
Japanese Patent JP3558294
Kind Code:
B2
Abstract:
Disclosed are devices and methods for the clinical analysis of a sperm sample. The devices comprise a solid substrate, typically on the order of a few millimeters thick and approximately 0.2 to 2.0 centimeters square, microfabricated to define a sample inlet port (16A) and a mesoscale flow channel (20, 40) extending from the inlet port (16A). In one embodiment, a sperm sample is applied to the inlet port (16A), and the competitive migration of the sperm sample through the mesoscale flow channel (20, 40) is detected to serve as an indicator of sperm motility. In another embodiment, the substrate of the device is microfabricated with a sperm inlet port (16A), an egg nesting chamber (16B), and an elongate mesoscale flow channel (20, 40) communicating between the egg nesting chamber (16B) and the inlet port (16A). In this embodiment, a sperm sample is applied to the inlet port, and the sperm in the sample is permitted to competitively migrate from the inlet port (16A) through the channel (20, 40) to the egg nesting chamber (16B), where in vitro fertilization occurs. The devices of the invention may be used in a wide range of applications in the analysis of a sperm sample, including the analysis of sperm morphology or motility, to assess sperm binding properties, and for in vitro fertilization.
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Inventors:
Wilding, Peter
Clicka, Larry Jay
Clicka, Larry Jay
Application Number:
JP51951793A
Publication Date:
August 25, 2004
Filing Date:
April 29, 1993
Export Citation:
Assignee:
Trustees of the University of Pennsylvania
International Classes:
B01D61/18; B01D67/00; B01D71/02; A01K67/02; B01J19/00; B01L3/00; B01L7/00; C12M1/00; C12M1/26; C12M1/34; C12M3/00; C12M3/08; C12N15/09; C12Q1/68; G01N11/04; G01N27/07; G01N33/483; G01N33/50; G01N33/543; G01N37/00; A61B17/04; B01F5/06; B01L9/00; C40B40/06; (IPC1-7): C12M1/00; C12M1/34; C12Q1/68
Domestic Patent References:
| JP3019700A | ||||
| JP3083572A | ||||
| JP63188399A | ||||
| JP63302366A |
Attorney, Agent or Firm:
Aoyama Aoi
Mitsuo Tanaka
Mitsuo Tanaka