Title:
ウシ種による組み換えポリペプチドの製造及びトランスジェニック法
Document Type and Number:
Japanese Patent JP3670003
Kind Code:
B2
Abstract:
A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the trangenic embryonic target cell formed thereby into a recipient bovine parent and indentifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk. The invention also includes transgenic bovine species as well as the milk from such transgenic bovine species. Methods are also provided for producing transgenic non-human mammals having a desirable phenotype. The method comprises first methylating a transgene followed by introduction into fertilized oocytes. The oocytes are then cultured to form pre-implantation embryons. Thereafter, at least one cell is removed from each of the pre-implantation embryons and the DNA digested with a restriction endonuclease capable of cleaving the methylated transgene but incapable of cleaving the unmethylated form of the transgene.
Inventors:
Debor, Hermann, A.
Striker, rain
Heineker, Herbert, Elle.
Platenburg, Gerald
Lee, Sang, F.
Peeper, Frank
Striker, rain
Heineker, Herbert, Elle.
Platenburg, Gerald
Lee, Sang, F.
Peeper, Frank
Application Number:
JP50179494A
Publication Date:
July 13, 2005
Filing Date:
June 15, 1993
Export Citation:
Assignee:
Farming bee buoy.
International Classes:
A01K67/027; A23C9/14; A23C9/20; A61K48/00; A61P15/00; C07H21/04; C07K14/47; C07K14/765; C07K14/79; C12N9/36; C12N9/64; C12N15/09; C12N15/85; C12P21/02; (IPC1-7): C12N15/09; A01K67/027; A23C9/14; C07K14/79
Foreign References:
| EP0451823A1 | ||||
| WO1991008216A1 |
Other References:
BIO/TECHNOLOGY, Vol.9, pp.844-847 (1991)
Nature Biotechnology, Vol.20.No.5,pp.484-487 (2002)
Nature Biotechnology, Vol.20.No.5,pp.484-487 (2002)
Attorney, Agent or Firm:
Hidesaku Yamamoto