Title:
分析される液体中の生物学的活性物質の測定方法
Document Type and Number:
Japanese Patent JP3708557
Kind Code:
B2
Abstract:
The present invention allows quickly, simply, precisely and with high sensitivity to determine the presence and concentration of any biologically active substances capable to interact with linear double-stranded DNA molecules in any liquids including biological liquids. The essence of the offered method consists that the lyotropic liquid-crystalline cholesteric DNA dispersion is formed in an aqueous-salted solution of a polymer, neutral in respect to DNA, of the linear double-stranded DNA molecules of a low molecular mass immediately before mixing with an analyzed liquid containing the determined substance, thereat the liquid for the analysis is prepared by mixing with the specified polymer under conditions, when the optical properties of the lyotropic liquid-crystalline DNA dispersion are not broken, then through the sample obtained as a result of mixing of the prepared analysed liquid with the liquid-crystalline DNA dispersion the circular-polarized light is passed, and the optical signal is registered at two wavelengths, one of them is in the region of the DNA absorption, and another one is in the region of absorption of the biologically active substance, then the ratio between these two signals is calculated, and concentration of the biologically active substance is determined on this ratio using the calibration curve. The offered device contains a wavelength selector (2) having an electrodynamic driver (22) of a positional type and at least one optical element (26, 27, 28) fixed on a motor (33) shaft (35) of the specified driver (22).
Inventors:
Evdukimov, Yuri Mikhailovich
Skridin, Sergey Gennadievich
Chernuja, Boris Alexandrovich
Mikhailov, Evgeni Leonidovich
Companets, Oleg Nikolaevich
Romanov, Sergei Nikolaevich
Korozov, Hradimill Basilievich
Skridin, Sergey Gennadievich
Chernuja, Boris Alexandrovich
Mikhailov, Evgeni Leonidovich
Companets, Oleg Nikolaevich
Romanov, Sergei Nikolaevich
Korozov, Hradimill Basilievich
Application Number:
JP52353598A
Publication Date:
October 19, 2005
Filing Date:
November 14, 1997
Export Citation:
Assignee:
Oor "Bioanaritic chessy Technologie"
International Classes:
G01N33/50; C07H21/00; G01N21/19; G01N21/31; (IPC1-7): G01N21/19; G01N33/50
Domestic Patent References:
| JP60165535A | ||||
| JP56154635A | ||||
| JP56043520A | ||||
| JP48052278A | ||||
| JP4190141A | ||||
| JP3130695A | ||||
| JP3130645A | ||||
| JP47003796B1 |
Foreign References:
| US3632216 | ||||
| US2586443 |
Other References:
Vysokomol Soedin Ser A Ser B, ロシア,1996年,Vol.38,No.5, p875-883
Liquid Crystals, 1995年,Vol.19,No.5, p595-602
Liquid Crystals, 1991年,Vol.9,No.2, p229-238
FEBS Letters, 1996年,Vol.392, p269-273
Liquid Crystals, 1995年,Vol.19,No.5, p595-602
Liquid Crystals, 1991年,Vol.9,No.2, p229-238
FEBS Letters, 1996年,Vol.392, p269-273
Attorney, Agent or Firm:
Takashi Ishida
Jun Tsuruta
Fukumoto product
Masaya Nishiyama
Higuchi Souji
Jun Tsuruta
Fukumoto product
Masaya Nishiyama
Higuchi Souji