Title:
異種多量体ポリペプチド類の製造方法
Document Type and Number:
Japanese Patent JP3775798
Kind Code:
B2
Abstract:
The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins, heteromultimers and antibody-immunoadhesin chimeras. Generally, the method involves introducing a protuberance at the interface of a first polypeptide and a corresponding cavity in the interface of a second polypeptide, such that the protuberance can be positioned in the cavity so as to promote heteromultimer formation and hinder homomultimer formation. The protuberance and cavity can be made by synthetic means such as altering the nucleic acid encoding the polypeptides or by peptide synthesis.
Inventors:
Carter, paul jay
Presta, Leonard G
Ridgway, John Bee
Presta, Leonard G
Ridgway, John Bee
Application Number:
JP52626096A
Publication Date:
May 17, 2006
Filing Date:
February 05, 1996
Export Citation:
Assignee:
Genentech Incorporated
International Classes:
C12N15/09; A61K38/00; A61K39/395; A61P31/04; A61P31/12; C07K14/73; C07K16/28; C07K16/46; C07K19/00; C12N5/10; C12N15/13; C12P21/08; C12R1/91
Domestic Patent References:
| JP7501698A |
Other References:
J Hematotherapy 4 p.389-394 (1995)
J Immunol 148(5) 1547-1553 (1992)
Protein Engineering 9(7) p.617-621 (1996)
Science 240 p.1759-1764 (1988)
J Immunol 148(5) 1547-1553 (1992)
Protein Engineering 9(7) p.617-621 (1996)
Science 240 p.1759-1764 (1988)
Attorney, Agent or Firm:
Masatake Shiga
Takashi Watanabe
Takashi Watanabe