Title:
高速自動化連続流、多次元分子選別および分析
Document Type and Number:
Japanese Patent JP4063687
Kind Code:
B2
Abstract:
The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second "dimension". The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
Inventors:
Jindal, Satish
Legnia, Fred, Yi.
Williams, Kevin
Afeyan, Nover, Bee.
Pariwar, Sandeep
David, Evans
Pingari, Aruna
Legnia, Fred, Yi.
Williams, Kevin
Afeyan, Nover, Bee.
Pariwar, Sandeep
David, Evans
Pingari, Aruna
Application Number:
JP2003031782A
Publication Date:
March 19, 2008
Filing Date:
February 07, 2003
Export Citation:
Assignee:
Perceptive Biosystems, Inc.
International Classes:
G01N27/62; G01N30/46; B01J20/281; C12Q1/68; C40B20/00; C40B30/04; C40B40/02; C40B50/06; C40B60/04; G01N27/64; G01N30/08; G01N30/50; G01N30/72; G01N30/84; G01N30/88; G01N33/15; G01N33/50; G01N33/543; G01N33/566; G01N30/02
Domestic Patent References:
| JP60097270A | ||||
| JP50141398A | ||||
| JP1156664A | ||||
| JP61030763A | ||||
| JP50046193A | ||||
| JP58200156A |
Attorney, Agent or Firm:
Kiyoshi Fujino