Title:
ポリペプチド変異体を調整するための方法
Document Type and Number:
Japanese Patent JP4156666
Kind Code:
B2
Abstract:
The present invention relates to a method for preparing positive polypeptide variants by shuffling different nucleotide sequences of homologous DNA sequences by in vivo recombination comprising the steps of a) forming at least one circular plasmid comprising a DNA sequence encoding a polypeptide, b) opening said circular plasmid(s) within the DNA sequence(s) encoding the polypeptide(s), c) preparing at least one DNA fragment comprising a DNA sequence homologous to at least a part of the polypeptide coding region on at least one of the circular plasmid(s), d) introducing at least one of said opened plasmid(s), together with at least one of said homologous DNA fragment(s) covering full-length DNA sequences encoding said polypeptide(s) or parts thereof, into a recombination host cell, e) cultivating said recombination host cell, and f) screening for positive polypeptide variants, wherein more than one cycle of step a) to f) are performed and/or wherein the shuffling is carried out with 2 or more DNA fragments with partially overlapping regions.
Inventors:
Okers, Jens, Sigart
Application Number:
JP50884197A
Publication Date:
September 24, 2008
Filing Date:
August 12, 1996
Export Citation:
Assignee:
Novozymes actiesel scab
International Classes:
C12N15/09; C12N1/19; C12N9/20; C12N15/10; C12P21/02; C12R1/01; C12R1/865
Domestic Patent References:
| JP1157383A |
Other References:
Gene, 1989年, vol.83, no.1, p.15-24
Nature, 1994年, vol.370, no.6488, p.389-391
Nature, 1994年, vol.370, no.6488, p.389-391
Attorney, Agent or Firm:
Takashi Ishida
Jun Tsuruta
Fukumoto product
Akemi Hino
Kazuhiro Nakamura
Yoichi Watanabe
Masato Ozeki
Masaya Nishiyama
Higuchi Souji
Jun Tsuruta
Fukumoto product
Akemi Hino
Kazuhiro Nakamura
Yoichi Watanabe
Masato Ozeki
Masaya Nishiyama
Higuchi Souji