Title:
タンパク質を含まない培地中での組み換え因子ＶＩＩＩの製造
Document Type and Number:
Japanese Patent JP4257685
Kind Code:
B2
Abstract:
Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymers, preferably in the presence of trace metals such as copper. In very preferred embodiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium.
Inventors:
キヤスリーン・ハリス
シヤム−ユエン・チヤン
シヤム−ユエン・チヤン
Application Number:
JP11590398A
Publication Date:
April 22, 2009
Filing Date:
April 13, 1998
Export Citation:
Assignee:
バイエル・コーポレーシヨンＢＡＹＥＲ ＣＯＲＰＯＲＡＴＩＯＮ
International Classes:
C12P21/02; C07K14/755; C12N5/00; C12N5/02; C12N5/10; C12P1/00; C12P21/04; C12R1/91
Attorney, Agent or Firm:
小田嶋 平吾
特許業務法人小田島特許事務所
特許業務法人小田島特許事務所