Title:
安定同位体標識アミノ酸とその標的蛋白質への組み込み方法、蛋白質のNMR構造解析方法並びに位置選択的安定同位体標識フマル酸と酒石酸の製造方法
Document Type and Number:
Japanese Patent JP4340538
Kind Code:
B2
Abstract:
The present invention provides a stable isotope-labeled amino acid which is at least one of amino acids constituting a protein and which has at least one of the following labeling patterns: (a) hydrogen atoms except at least one hydrogen atom in one or more methylene groups are deuterated, (b) hydrogen atoms in one of prochiral gem-methyl groups are completely deuterated, (c) hydrogen atoms in prochiral methyl groups are partially deuterated, and (d) all hydrogen atoms except one of them in methyl group are deuterated and hydrogen atoms in the aromatic ring are partially deuterated. With the stable isotope-labeled amino acid, the deuteration of protein can be attained without damaging the NMR sensitivity of remaining hydrogen nucleus and, in addition, the rapid, accurate analysis of NMR spectrum of a high-molecular protein which is beyond the limitation in the prior art and the determination of the stereo-structure can be performed at the same time.
Inventors:
Kaiso Masatsune
Tsutomu Terauchi
Tsutomu Terauchi
Application Number:
JP2003554627A
Publication Date:
October 07, 2009
Filing Date:
December 19, 2002
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
C07C229/08; C07B59/00; C07C51/09; C07C229/22; C07C229/24; C07C229/26; C07C229/36; C07C323/58; C07K1/13; C12P21/02; G01N24/08
Domestic Patent References:
| JP5236986A |
Foreign References:
| WO1999011589A1 |
Other References:
Journal of the American Chemical Society,1978年,Vol.100, No.1,p.54-58
Journal of the Chemical Society, Perkin Transactions 1,2000年,No.20,p.3406-3416
Journal of Biomolecular NMR,1994年,Vol.4, No.3,p.335-340
Journal of Biomolecular NMR,2000年,Vol.18, No.1,p.75-76
Journal of the Chemical Society, Perkin Transactions 1,2000年,No.20,p.3406-3416
Journal of Biomolecular NMR,1994年,Vol.4, No.3,p.335-340
Journal of Biomolecular NMR,2000年,Vol.18, No.1,p.75-76
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama