Title:
アルツハイマー病及びプリオン病の治療に関する抗プラーク療法を特定するコンゴ好染性マルタ十字形アミロイドプラークのinvitroにおける形成
Document Type and Number:
Japanese Patent JP4537577
Kind Code:
B2
Abstract:
Co-incubation of an amyloid protein with sulfated macromolecules as a method for the formation of amyloid plaques. The amyloid protein may be the beta-amyloid protein or the prion protein or the like. Amyoid plaque formation in one embodiment proceeds in vitro and desirably produces amyloid plaques that stain with Congo red and demonstrate a maltese-cross pattern when viewed under polarized light. The method also produces amyloid plaques that demonstrate an "amyloid star" appearance when viewed by transmission electron microscopy. An in vivo assay is also presented for selecting a candidate therapeutic agent for inhibiting or disrupting amyloid plaque deposition or persistence.
Inventors:
Castillo, Gerrard
Snow, alan dee
Snow, alan dee
Application Number:
JP2000535360A
Publication Date:
September 01, 2010
Filing Date:
March 12, 1999
Export Citation:
Assignee:
UNIVERSITY OF WASHINGTON
International Classes:
A61K38/00; G01N33/50; A01K67/027; A61K47/30; A61K49/00; A61P25/28; A61P43/00; C07K1/10; C07K14/47; G01N33/15; G01N33/53
Other References:
Alan D. Snow,An Important Role of Heparan Sulfate Proteoglycan (Perlecan) in a Model System for the Deposition and Persistence of Fibrillar Aβ-Amyloid in Rat Brain,Neuron,1994年,Vol.12,Pages 219-234
Gerardo M. Castillo,Perlecan Binds to the β-Amyloid Proteins (Aβ) of Alzheimer's Disease, Accelerates Aβ Fibril Formation, and Maintains Aβ Fibril Stability,Journal of Neurochemistry,1997年,Vol.69, No.6,Pages 2452-2465
Karen J. Bame,Aβ(1-40) Prevents Heparanase-catalyzed Degradation of Heparan Sulfate Glycosaminoglycans and Proteoglycans in Vitro,Journal of Biological Chemistry,1997年,Vol.272, No.27,Pages 17005-17011
Kurt R. Brunden,pH-Dependent Binding of Synthetic β-Amyloid Peptides to Glycosaminoglycans,Journal of Neurochemistry,1993年,Vol.61, No.6,Pages 2147-2154
B. Leveugle,Binding of heparan sulfate glycosaminoglycan to β-amyloid peptide: inhibition by potentially therapeutic polysulfated compounds,NeuroReport,1994年,Vol.5, No.11,Page 1389-1392
GM Castillo,The Glycosaminoglycans of Perlecan Bind Beta-Amyloid Protein (AB) and Enhance AB Fibril Formation in a Sulfate Content Dependent Manner.,Brain Pathology,1997年,Vol.7, No.4,Page 1369
Gerardo M. Castillo,Perlecan Binds to the β-Amyloid Proteins (Aβ) of Alzheimer's Disease, Accelerates Aβ Fibril Formation, and Maintains Aβ Fibril Stability,Journal of Neurochemistry,1997年,Vol.69, No.6,Pages 2452-2465
Karen J. Bame,Aβ(1-40) Prevents Heparanase-catalyzed Degradation of Heparan Sulfate Glycosaminoglycans and Proteoglycans in Vitro,Journal of Biological Chemistry,1997年,Vol.272, No.27,Pages 17005-17011
Kurt R. Brunden,pH-Dependent Binding of Synthetic β-Amyloid Peptides to Glycosaminoglycans,Journal of Neurochemistry,1993年,Vol.61, No.6,Pages 2147-2154
B. Leveugle,Binding of heparan sulfate glycosaminoglycan to β-amyloid peptide: inhibition by potentially therapeutic polysulfated compounds,NeuroReport,1994年,Vol.5, No.11,Page 1389-1392
GM Castillo,The Glycosaminoglycans of Perlecan Bind Beta-Amyloid Protein (AB) and Enhance AB Fibril Formation in a Sulfate Content Dependent Manner.,Brain Pathology,1997年,Vol.7, No.4,Page 1369
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Kiyoshi Murakami
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Kiyoshi Murakami