Title:
質量分析計を使った膜タンパク質の定量方法
Document Type and Number:
Japanese Patent JP4670060
Kind Code:
B2
Abstract:
The present invention is to provide a method for quantifying a plasma membrane protein present in a cell membrane, by using a stable-isotope labeled peptide as a probe by mass spectrometry, in a simple, quickly and accurate manner. A plasma membrane protein is fragmented to prepare an oligopeptide fragment, identified by LC-MSMS, and by setting as essential criteria that the peptide is obtained by fragmentating with a protease, and the peptide is specific to a target molecule, selective criteria with score are set for hydrophobic amino acids content, sequence conditions, number of amino acid residues, specific amino acid sequence conditions, etc. According to criterion for selecting a subject peptide for quantification for selecting preferentially a peptide with high score, a peptide fragment that can be ionized by ESI method is selected, and by using the subject peptide for quantification and a stable-isotope labeled peptide, the plasma membrane protein is quantified accurately by mass spectrometry.
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Inventors:
Junichi Kamie
Juno Otsuki
Tetsuya Terasaki
Juno Otsuki
Tetsuya Terasaki
Application Number:
JP2007544097A
Publication Date:
April 13, 2011
Filing Date:
October 27, 2006
Export Citation:
Assignee:
Tohoku University
International Classes:
G01N27/62
Domestic Patent References:
| JP2002524755A | 2002-08-06 | |||
| JP2002515820A | 2002-05-28 | |||
| JP2005185281A | 2005-07-14 |
Foreign References:
| WO2004002996A1 | 2004-01-08 |
Attorney, Agent or Firm:
Masaki Hirota