Title:
発酵乳酸桿菌のN-デオキシリボシルトランスフェラーゼと、その酵素を利用した2’,3’-ジデオキシヌクレオシドと2’,3’-ジデヒドロ-2’,3’-ジデオキシヌクレオシドの合成
Document Type and Number:
Japanese Patent JP4690390
Kind Code:
B2
Abstract:
N-deoxyribosyl transferases of Lactobacillus fermentum and their analogues, as well their use for the enzymatic synthesis of 2′,3′-dideoxynucleosides and 2′,3′-didehydro-2′,3′-dideoxynucleosides. These transferases and their analogues include a N-deoxyribosyl transferase protein (DTP) that has at least 70%-95% identity with the polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, that retains residues Y13, D77, D97, E103, and M312 which respectively correspond to positions 13, 77, 97, 103, and 132 of SEQ ID NO: 2; and that has threonine at a position corresponding to position 15 of SEQ ID NO: 2 or SEQ ID NO: 4. Polynucleotides, vectors and host cells encoding these N-deoxyribosyl transferases and their analogues.
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Inventors:
Kaminski, Pierre-Alexandre
Application Number:
JP2007505586A
Publication Date:
June 01, 2011
Filing Date:
March 29, 2005
Export Citation:
Assignee:
INSTITUT PASTEUR
International Classes:
C12N15/09; A61K31/7088; A61P31/00; A61P35/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P19/38; C12P19/40
Domestic Patent References:
| JP2005503159A |
Foreign References:
| WO2003025163A1 |
Other References:
Bioorganic & Medicinal Chemistry Letters, (1993), Vol. 3, No. 6, p. 1185-1188
Current Opinion in Biotechnology, (2000), Vol. 11, p. 209-214
Biocatalysis and Biotransformation, (2001), Vol. 19, p. 85-97
Current Opinion in Biotechnology, (2000), Vol. 11, p. 209-214
Biocatalysis and Biotransformation, (2001), Vol. 19, p. 85-97
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Rieko Koshisakabe
Takashi Ishida
Fukumoto product
Tetsuji Koga
Rieko Koshisakabe