Title:
原核宿主における組換えグリコシル化タンパク質産生のためのシステムおよび方法
Document Type and Number:
Japanese Patent JP4727927
Kind Code:
B2
Abstract:
A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.
Inventors:
Marx Ebi
Michael Wacker
Michael Wacker
Application Number:
JP2003573140A
Publication Date:
July 20, 2011
Filing Date:
March 05, 2003
Export Citation:
Assignee:
Eidgenoessische Technische Hochschule Zuerich
International Classes:
A23L1/305; C12N1/21; A61K8/64; A61K8/96; A61K38/00; A61K38/16; A61K39/00; A61K47/48; C07H21/04; C07K16/12; C12N9/10; C12N15/09; C12P21/00; C12P21/02; C12P21/06; C12R1/19
Domestic Patent References:
| JP6181771A | ||||
| JP9501044A |
Foreign References:
| CA2360205A1 |
Other References:
生物と化学,2001,Vol.39,No.10,p.632-634
Attorney, Agent or Firm:
Mitsuo Tanaka
Michiko Matsutani
Tadayuki Motoyama
Kenji Tomita
Mina Shiga
Michiko Matsutani
Tadayuki Motoyama
Kenji Tomita
Mina Shiga