Title:
位置特定可能なアレイによるリガーゼ検出反応を用いた核酸配列差異の検出
Document Type and Number:
Japanese Patent JP4766750
Kind Code:
B2
Abstract:
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
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Inventors:
Ballany Francis
Jerry Norman Pee.
Witowski Nancy E.
Day joseph
Hammer Robert P.
Ballany George
Jerry Norman Pee.
Witowski Nancy E.
Day joseph
Hammer Robert P.
Ballany George
Application Number:
JP2000606786A
Publication Date:
September 07, 2011
Filing Date:
March 17, 2000
Export Citation:
Assignee:
Cornell Research Foundation, Inc.
Regents of the University of Minnesota
BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE
Regents of the University of Minnesota
BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE
International Classes:
C12N15/09; G01N33/53; C12Q1/68; C12Q1/6827; C12Q1/6837; C12Q1/6858; G01N33/566; G01N37/00; C40B40/06; C40B50/08; C40B60/14
Foreign References:
| WO1997031256A2 | 1997-08-28 | |||
| WO1998003673A1 | 1998-01-29 |
Attorney, Agent or Firm:
Hatsushi Shimizu