Title:
ZAP-70相互作用分子の同定およびZAP-70の精製のための方法
Document Type and Number:
Japanese Patent JP4987970
Kind Code:
B2
Abstract:
The invention provides in a first aspect a method for the identification of an ZAP-70 interacting compound, comprising the steps of a) providing a protein preparation containing phosphorylated ZAP-70, b) contacting the protein preparation with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex, c) incubating the aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex with a given compound, and d) determining whether the compound is able to separate phosphorylated ZAP-70 from the immobilized aminopyrido-pyrimidine ligand 24. In a second aspect, the present invention relates to A method for the identification of an ZAP-70 interacting compound, comprising the steps of a) providing a protein preparation containing phosphorylated ZAP-70, b) contacting the protein preparation with ligand aminopyrido-pyrimidine ligand 24 immobilized on a solid support and with a given compound under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex, and c) detecting the aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex formed in step b). In a third aspect, the invention provides a method for the identification of an ZAP-70 interacting compound, comprising the steps of: a) providing two aliquots of a protein preparation containing phosphorylated ZAP-70, b) contacting one aliquot with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex, c) contacting the other aliquot with aminopyrido-pyrimidine ligand 24 immobilized on a solid support and with a given compound under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex, and d) determining the amount of aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex formed in steps b) and c). In a forth aspect, the invention relates to a method for the identification of an ZAP-70 interacting compound, comprising the steps of: a) providing two aliquots comprising each at least one cell containing phosphorylated ZAP-70, b) incubating one aliquot with a given compound, c) harvesting the cells of each aliquot, d) lysing the cells in order to obtain protein preparations, e) contacting the protein preparations with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex, and f) determining the amount of aminopyrido-pyrimidine ligand 24 - phosphorylated ZAP-70 complex formed in each aliquot in step e).
More Like This:
| WO/1998/021580 | VITAMIN D IMMUNOASSAY SYSTEMS |
| JP2005065617 | METHOD FOR IDENTIFYING INDIVIDUAL |
| JP6726107 | Systems and methods for electronic biological sample analysis |
Inventors:
Cruise willich
Ramsden Nigel
Drews Gerald
Ebelhard Dirk
Ramsden Nigel
Drews Gerald
Ebelhard Dirk
Application Number:
JP2009512504A
Publication Date:
August 01, 2012
Filing Date:
June 01, 2007
Export Citation:
Assignee:
Cellsome AG
International Classes:
G01N33/53; G01N27/62; G01N33/15; G01N33/50; A61P29/00; A61P37/06; C12N15/09
Domestic Patent References:
| JP2009515170A | ||||
| JP2008539794A | ||||
| JP2002326990A |
Foreign References:
| WO2004081531A1 |
Attorney, Agent or Firm:
Hatsushi Shimizu
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki