Title:
多能性幹細胞を増殖させる方法
Document Type and Number:
Japanese Patent JP5464443
Kind Code:
B2
Abstract:
For efficient proliferation of pluripotent stem cells in a system free from any animal-derived material such as feeder cells or serum, the present invention provides a method for proliferation of pluripotent stem cells, which comprises culturing the pluripotent stem cells in a medium free from both feeder cells and serum in a system containing laminin-5.
Inventors:
Naotaka Yasuda
Munehiro Yamada
Miyazaki incense
Takahashi Kazutori
Munehiro Yamada
Miyazaki incense
Takahashi Kazutori
Application Number:
JP2010506007A
Publication Date:
April 09, 2014
Filing Date:
March 31, 2009
Export Citation:
Assignee:
Oriental Yeast Industry Co., Ltd.
Kyoto University
Kyoto University
International Classes:
C12N5/0735; C07K14/47; C12N5/00; C12N15/09
Foreign References:
WO2007023875A1 | 2007-03-01 | |||
US20050233446A1 | 2005-10-20 | |||
WO2006029198A2 | 2006-03-16 | |||
WO1998030679A1 | 1998-07-16 |
Other References:
JPN6009032280; HASHIMOTO J et al.: 'Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by lami' Stem Cells vol. 24, 2006, p. 2346-2354
JPN6009032283; LI Y et al.: 'Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived produc' Biotechnol. Bioeng. vol. 91, 2005, p. 688-698
JPN6009032283; LI Y et al.: 'Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived produc' Biotechnol. Bioeng. vol. 91, 2005, p. 688-698
Attorney, Agent or Firm:
Shinjiro Ono
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Reiko Izumiya
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Reiko Izumiya