Title:
RNA干渉のRNA配列特異的メディエータ
Document Type and Number:
Japanese Patent JP5500750
Kind Code:
B2
Abstract:
The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function.; The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications.
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Inventors:
Toushur, Thomas
Sharp, Philip, A.
The More, Philip, Dee.
Bartel, David, Pea.
Sharp, Philip, A.
The More, Philip, Dee.
Bartel, David, Pea.
Application Number:
JP2001573036A
Publication Date:
May 21, 2014
Filing Date:
March 30, 2001
Export Citation:
Assignee:
Whitehead Institute Fore Biomedical Research
Max-Plank-Gezelle Shaft Tool Forderung Del Wissenschafften A.V.
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
University of Massachusetts
Max-Plank-Gezelle Shaft Tool Forderung Del Wissenschafften A.V.
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
University of Massachusetts
International Classes:
C12N15/09; A61K31/7105; C12N15/113; A61K48/00; A61P43/00; C07H21/00; C12N1/00; C12N5/10; C12N15/10; C12Q1/02; C12Q1/68; A61K38/00; C07H; C12N
Domestic Patent References:
| JP2003502012A |
Foreign References:
| WO2000044895A1 | ||||
| US6107094 |
Other References:
Science, 286[5441] (1999) p.950-952
Genes Dev., 13[24](1999) p.3191-3197
Nature, 404[6775](2000Mar16) p.293-296
Nature, 409[6818](2001Jan18) p.363-366
Mol. Cell. Biol., 12[11](1992) p.5238-5248
Nature, 404(2000Mar16) p.293-296
Genes Dev., 13[24](1999) p.3191-3197
Nature, 404[6775](2000Mar16) p.293-296
Nature, 409[6818](2001Jan18) p.363-366
Mol. Cell. Biol., 12[11](1992) p.5238-5248
Nature, 404(2000Mar16) p.293-296
Attorney, Agent or Firm:
Yoshinori Hosoda