Title:
リボ核酸を検出するための組成物、方法およびキット
Document Type and Number:
Japanese Patent JP5685085
Kind Code:
B2
Abstract:
Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.
Inventors:
キュエルスティン， アール． スコット
Application Number:
JP2010542413A
Publication Date:
March 18, 2015
Filing Date:
January 13, 2009
Export Citation:
Assignee:
アプライド バイオシステムズ リミテッド ライアビリティー カンパニー
International Classes:
C12Q1/68; C12N15/09
Attorney, Agent or Firm:
Shusaku Yamamoto
Natsuki Morishita
Natsuki Morishita