Title:
真核生物細胞を改変する方法
Document Type and Number:
Japanese Patent JP5692863
Kind Code:
B2
Abstract:
A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
Inventors:
Andrew Jay Murphy
George Dee. Yongkopoulos
David Valenzuela
Margaret Callow
Lynn McDonald
Shawn Stevens
Alice Economize
George Dee. Yongkopoulos
David Valenzuela
Margaret Callow
Lynn McDonald
Shawn Stevens
Alice Economize
Application Number:
JP2012032592A
Publication Date:
April 01, 2015
Filing Date:
February 17, 2012
Export Citation:
Assignee:
REGENERON PHARMACEUTICALS, INC.
International Classes:
A01K67/027; C12N15/09; C07K16/00; C07K16/46; C12N1/15; C12N1/19; C12N5/02; C12N5/08; C12N5/10; C12N15/00; C12N15/79; C12N15/90; C12P21/02; C12P21/04; C12P21/08; C12Q1/02; C12Q1/68
Domestic Patent References:
| JP7509137A |
Other References:
Yang XW, Model P, Heintz N.,Homologous recombination based modification in Escherichia coli and germline transmission in transge,Nat Biotechnol.,1997年 9月,Vol.15, No.9,p.859-865
Deng C, Capecchi MR.,Reexamination of gene targeting frequency as a function of the extent of homology between the target,Mol Cell Biol.,1992年 8月,Vol.12, No.8,p.3365-3371
Deng C, Capecchi MR.,Reexamination of gene targeting frequency as a function of the extent of homology between the target,Mol Cell Biol.,1992年 8月,Vol.12, No.8,p.3365-3371
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita
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