Title:
方法
Document Type and Number:
Japanese Patent JP5774612
Kind Code:
B2
Abstract:
A process is provided for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence and obtaining the albumin, wherein the cell has a genetic modification which causes the cell to have at least a reduced capacity of mannosylation of the recombinantly-expressed albumin and wherein the culture medium is at least 1,000L and is of pH 5.3 to 6.8. A process for the preparation of a highly pure albumin solution is disclosed, the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons.
Inventors:
Hendrick, van, arc
David, John, Mead
Philip, Harvey, Morton
Andrew, John, Cartwright
Jason, Cameron
David, James, Balance
Michelle, Gaston, Joseph, Grand Georges
Stephen, Beletenko
John, Rodney, Woodrow
Darrell, sleep
Jean-Luc, Bernard, Belon
David, John, Mead
Philip, Harvey, Morton
Andrew, John, Cartwright
Jason, Cameron
David, James, Balance
Michelle, Gaston, Joseph, Grand Georges
Stephen, Beletenko
John, Rodney, Woodrow
Darrell, sleep
Jean-Luc, Bernard, Belon
Application Number:
JP2013004867A
Publication Date:
September 09, 2015
Filing Date:
January 15, 2013
Export Citation:
Assignee:
Novozymes By Offer Madeco Actiezel Scab
International Classes:
C07K1/16; G01N33/53; A61K38/00; A61P7/08; A61P17/02; C07K1/18; C07K1/22; C07K1/34; C07K14/765; C12N1/19; C12N15/09; C12N15/14; C12P21/02; G01N30/00; C12R1/865
Foreign References:
| WO1997031947A1 |
Attorney, Agent or Firm:
Hirohito Katsunuma
Yukitaka Nakamura
Noritaka Yokota
Yuko Matsuyama
Yukitaka Nakamura
Noritaka Yokota
Yuko Matsuyama