Title:
皮膚の加齢を防止および／もしくは減弱し、並びに／または皮膚に潤いを与えるのに用いるためのマイクロＲＮＡ阻害剤
Document Type and Number:
Japanese Patent JP5886278
Kind Code:
B2
Abstract:
The invention relates to the identification and the use of compounds which inhibit the expression or activity of micro-RNAs for preventing and/or attenuating ageing, and/or for hydrating skin. The invention thus relates to an in vitro method for screening for candidate compounds for preventing and/or attenuating ageing of the skin, and/or for hydrating the skin, comprising the following steps:
a. bringing at least one test compound in contact with a sample of keratinocytes ;
b. measuring the expression or the activity of at least one microRNA in said keratinocytes;
c. selecting the compounds for which an inhibition of at least 20%, preferably at least 30%, preferably at least 40 % of the expression or an inhibition of at least 20%, preferably at least 30%, preferably at least 40 % of the activity of at least one microRNA is measured in the keratinocytes treated in a. compared with the untreated keratinocytes.
Inventors:
Kandy, Eleonora
Merino, Gennaro
Santini, frog
Mahe, Christian
Merino, Gennaro
Santini, frog
Mahe, Christian
Application Number:
JP2013513665A
Publication Date:
March 16, 2016
Filing Date:
June 07, 2011
Export Citation:
Assignee:
Chanel Perfumes Butte
International Classes:
C12Q1/02; A61K45/00; A61Q19/08; C12N5/10; C12Q1/68
Domestic Patent References:
| JP2008519606A | ||||
| JP2005505392A | ||||
| JP2009051790A | ||||
| JP2009185007A | ||||
| JP2005509456A |
Foreign References:
| WO2009066967A1 | ||||
| WO2009051912A1 | ||||
| WO2010006412A1 | ||||
| US20040224892 |
Other References:
Cell Death and Differentiation,2008年,Vol.15,pp.1187-1195
Genes & Development,2005年,Vol.19,pp.1986-1999
Journal of Bioscience and Bioengineering,2002年,Vol.94, No.3,pp.231-236
Biochemical and Biophysical Research Communications,2010年12月,Vol.404,pp.896-902
Genes & Development,2005年,Vol.19,pp.1986-1999
Journal of Bioscience and Bioengineering,2002年,Vol.94, No.3,pp.231-236
Biochemical and Biophysical Research Communications,2010年12月,Vol.404,pp.896-902
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Kyoko Tamura
Yasuhito Suzuki
Norio Omori
Kyoko Tamura
Yasuhito Suzuki