Title:
ジフテリア毒素−インターロイキン−３コンジュゲートに基づく方法及び組成物
Document Type and Number:
Japanese Patent JP5902223
Kind Code:
B2
Abstract:
The present invention provides methods for inhibiting interleukin-3 receptor-expressing cells, and, in particular, inhibiting the growth of such cells by using a diphtheria toxin-human interleukin-3 conjugate (DT-IL3) that is toxic to cells expressing the interleukin-3 receptor. In preferred embodiments, the DT-IL3 conjugate is a fusion protein comprising amino acids 1-388 of diphtheria toxin fused via a peptide linker to full-length, human interleukin-3. In certain embodiments, the methods of the present invention relate to the administration of a DT-IL3 conjugate to inhibit the growth of cancer cells and/or cancer stem cells in humans, which cells express one or more subunits of the interleukin-3 receptor. Exemplary cells include myeloid leukemia cancer stem cells. In other embodiments, the methods of the present invention relate to ex vivo purging of bone marrow or peripheral blood to remove cells that express one or more subunits of the interleukin-3 receptor such that the purged bone marrow or peripheral blood is suitable, e.g., for autologous stem cell transplantation to restore hematopoietic function.
Inventors:
Alto Fruity Frankel
Application Number:
JP2014075847A
Publication Date:
April 13, 2016
Filing Date:
April 01, 2014
Export Citation:
Assignee:
Scott and White Healthcare
International Classes:
A61K38/00; A61K35/74; A61P7/00; A61P35/02
Other References:
Blood,2003年,vol.102,no.11(part 1 of 2),386a-511I
Protein Engineering,1999年,vol.12,no.9,p.779-785
Blood,1997年,vol.90,no.10(part 1 of 2),187a-828
Blood,2001年,vol.98,no.11(part 1 of 2),328a 491-II
Protein Engineering,1999年,vol.12,no.9,p.779-785
Blood,1997年,vol.90,no.10(part 1 of 2),187a-828
Blood,2001年,vol.98,no.11(part 1 of 2),328a 491-II
Attorney, Agent or Firm:
Toru Ishikawa