増強された検出特性を有する変異プロテアーゼバイオセンサー

Japanese Patent JP5961163

B2

A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to an unmodified circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the unmodified circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

Vinco Ski Block
Butler Braden
Encel Lance
Fan frank
Hook blood
Otto pole
Vidugiris Gediminas
Wigdal Susan
Zimmerman Christopher

JP2013510280A

August 02, 2016

May 11, 2011

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Promega Corporation

C12N15/09; A01K67/027; A01K67/033; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12Q1/66

JP2009532063A
JP2003509051A

WO2009049892A1
WO2008030968A1

Current Chemical Genomics, 2008, Vol.2, pp.16-28
ACS Chemical Biology, 2008, Vol.3, No.6, pp.346-351

Koichi Tsujii
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Shimura

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