Title:
キメラ２重鎖核酸
Document Type and Number:
Japanese Patent JP6416301
Kind Code:
B2
Abstract:
A method of reducing the level of a transcription product in a cell comprising contacting with the cell a composition comprising a double-stranded nucleic acid complex comprising a first nucleic acid strand annealed to a second nucleic acid strand, wherein: (i) the first nucleic acid strand hybridizes to the transcription product and comprises (a) a region consisting of at least 4 consecutive nucleotides that are recognized by RNase H when the strand is hybridized to the transcription product, (b) one or more nucleotide analogs located on 5′ terminal side of the region, (c) one or more nucleotide analogs located on 3′ terminal side of the region and (d) a total number of nucleotides and nucleotide analogs ranging from 8 to 35 nucleotides and (ii) the second nucleic acid strand comprises (a) nucleotides and optionally nucleotide analogs and (b) at least 4 consecutive RNA nucleotides.
Inventors:
Takanori Yokota
Kazutaka Nishina
Satoshi Kobiga
Mizusawa Hidehiro
Kazutaka Nishina
Satoshi Kobiga
Mizusawa Hidehiro
Application Number:
JP2017044034A
Publication Date:
October 31, 2018
Filing Date:
March 08, 2017
Export Citation:
Assignee:
Tokyo Medical And Dental University, Medical Hospital
National University Corporation Osaka University
National University Corporation Osaka University
International Classes:
C12N15/113
Domestic Patent References:
| JP2010500003A | ||||
| JP2006522586A | ||||
| JP9110894A |
Foreign References:
| WO2008111908A1 | ||||
| WO2005113571A1 |
Other References:
Database GenBank [online], Accessin No. NM_018963, 03-SEP-2011 updated,[retrieved on 2018-02-22]DUBINSKY, M. C. et al.,Definition: Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Satoshi Fujita
Natsuo Tanaka