Title:
キナーゼを用いた新規な測定方法及び組成物
Document Type and Number:
Japanese Patent JP6450394
Kind Code:
B2
Abstract:
The present invention provides a measuring method for at least one of a kinase forward reaction substrate, a phosphorylated product thereof, and a precursor thereof, and includes a step of conducting an enzymatic cycling reaction by bringing at least a kinase, a first nucleotide coenzyme of the kinase, and a second nucleotide coenzyme having a different nucleoside moiety from the first nucleotide coenzyme into contact with a sample; a step of detecting a signal corresponding to a change of at least one of the first nucleotide coenzyme and a conversion product thereof, and the second nucleotide coenzyme and a conversion product thereof; and (3) a step of calculating, on the basis of the detected change of the signal, an amount of the kinase forward reaction substrate and/or the phosphorylated product thereof contained in the sample.
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Inventors:
Ueda Shigeru
Shinichi Sakasegawa
Shinichi Sakasegawa
Application Number:
JP2016550299A
Publication Date:
January 09, 2019
Filing Date:
September 18, 2015
Export Citation:
Assignee:
Asahi Kasei Pharma Co., Ltd.
International Classes:
C12Q1/48; C12Q1/26; C12Q1/32; C12Q1/34; C12Q1/54
Domestic Patent References:
| JP60047698A | ||||
| JP2006223163A | ||||
| JP9187295A |
Foreign References:
| WO2011056611A1 |
Other References:
The Journal of Biological Chemistry,1986年,Vol. 261, No. 18,pp. 8249-8253
Journal of the American Chemical Society,1998年,Vol. 120, No. 28,pp. 6851-6858
Journal of the American Chemical Society,1998年,Vol. 120, No. 28,pp. 6851-6858
Attorney, Agent or Firm:
Yoshiyuki Inaba
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito