Title:
核酸増幅法
Document Type and Number:
Japanese Patent JP6489017
Kind Code:
B2
Abstract:
The purpose of the present invention is to provide a method for efficiently amplifying a target nucleic acid in a PCR containing a base analog. A method for amplifying nucleic acid using a reaction composition containing a base analog, wherein the reaction composition contains (a) a variant of DNA polymerase belonging to the B family having reduced base analog detection activity, and (b) a PCNA that exhibits amplification and reinforcement activity.
Inventors:
Satoru Kobayashi
Hiroshi Matsumoto
Hiroshi Matsumoto
Application Number:
JP2015530853A
Publication Date:
March 27, 2019
Filing Date:
August 01, 2014
Export Citation:
Assignee:
Toyobo Co., Ltd.
International Classes:
C12N15/09; C12Q1/48; C12Q1/686
Domestic Patent References:
| JP2006513726A | ||||
| JP2006521112A | ||||
| JP2002360261A | ||||
| JP2014079236A | ||||
| JP2014079237A | ||||
| JP2014079235A |
Foreign References:
| WO2007016702A1 | ||||
| WO2007004654A1 |
Other References:
EMPTAGE, K. et al.,"Interplay between DNA polymerase and proliferating cell nuclear antigen switches off base excision repair of uracil and hypoxanthine during replication in archaea.",J. MOL. BIOL.,2008年11月21日,Vol.383, No.4,P.762-771