Title:
ポリペプチドの産生の方法
Document Type and Number:
Japanese Patent JP6661549
Kind Code:
B2
Abstract:
The current invention reports a promoter that has the nucleic acid sequence of SEQ ID NO: 02 or SEQ ID NO: 03 which is a human CMV major immediate-early (hCMV-MIE) promoter/enhancer with C to G point mutation at position −41 and/or −179 relative to the transcription start site. This new promoter is especially useful for the production of polypeptides at large scale as it shows reduced promoter silencing and improved polypeptide production.
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Inventors:
Goepfeld Ulrich
Moritz Benjamin
Moritz Benjamin
Application Number:
JP2016568645A
Publication Date:
March 11, 2020
Filing Date:
May 18, 2015
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12N5/10; C12N15/11; C12N15/85; C12P21/02; C12P21/08
Foreign References:
| WO2012099540A1 |
Other References:
Osterlehner, A., et al.,"Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines",Biotechnol. bioeng.,2011年,Vol. 108,pp. 2670-2681
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto