Title:
稀少突然変異の検出方法、検出装置及びコンピュータプログラム
Document Type and Number:
Japanese Patent JP6679065
Kind Code:
B2
Abstract:
Disclosed is a method for detecting a rare mutation. The method comprises: preparing a sample comprising not more than 1,000 copies of template DNA; amplifying the template DNA to prepare a library, and analyzing a nucleotide sequence of the library; calculating a ratio of variants in a base at a predetermined position, from the analysis result; comparing the calculated ratio of variants with a predetermined cut-off value; and determining that the sample has a rare mutation in the base at the predetermined position when the calculated ratio of variants is not less than the predetermined cut-off value.
Inventors:
Toshikazu Ushijima
Satoshi Yamashita
Satoshi Yamashita
Application Number:
JP2015199342A
Publication Date:
April 15, 2020
Filing Date:
October 07, 2015
Export Citation:
Assignee:
National Cancer Research Center
Sysmex Corporation
Sysmex Corporation
International Classes:
C12Q1/6869; G06F17/00
Foreign References:
| WO2007050465A1 |
Other References:
BMC Genomics, 2015.03.24, Vol. 16, No. 229, pp. 1-15
Attorney, Agent or Firm:
Shintaro Nogawa
Shinji Kai
Yusuke Kaneko
Kiyoshi Inamoto
Shinji Kai
Yusuke Kaneko
Kiyoshi Inamoto