Title:
タグ付抗体
Document Type and Number:
Japanese Patent JP6744670
Kind Code:
B2
Abstract:
To provide techniques for enhancing Fab antibody forming efficiency and uses thereof.SOLUTION: An antibody H chain gene encoding a VH region and a CH1 region and an antibody L chain gene encoding a VL region and a CL region are co-expressed to obtain a Fab antibody. Alternatively, an antibody H chain gene encoding a VH region and a CH1 region and an antibody L chain gene encoding a VL region and a CL region are separately expressed and the expression products are mixed to obtain a Fab antibody. A first tag sequence encoding one peptide of a peptide pair forming a leucine zipper is added to the H chain gene and a second tag sequence encoding the other peptide of the leucine zipper peptide pair is added to the L chain gene.SELECTED DRAWING: None
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Inventors:
Hideo Nakano
Takaaki Kojima
Akiyo Kato
Takaaki Kojima
Akiyo Kato
Application Number:
JP2019047851A
Publication Date:
August 19, 2020
Filing Date:
March 15, 2019
Export Citation:
Assignee:
Tokai National University Organization
International Classes:
C12P21/08; C07K16/00; C07K19/00
Foreign References:
| WO2003018749A2 | ||||
| WO2001000814A2 |
Other References:
J. Mol. Biol.,2001年,vol.312, no.1,pp.221-228
Attorney, Agent or Firm:
Seiji Matsumoto