多能性細胞を肝細胞系統の細胞へと分化させるためのラミニンの使用 - Anselm (Anstitut Nacional de la Sante et de la Lecherche Medical)

Title:

多能性細胞を肝細胞系統の細胞へと分化させるためのラミニンの使用

Document Type and Number:

Japanese Patent JP6840084

Kind Code:

B2

Abstract:

The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human fetal hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4± and not expressing or substantially not expressing AFP for use in a method of treatment of the human body.

More Like This:

JP2017201899	METHOD FOR PRODUCING CELL FOR SCREENING FOR CONFORMATION DISEASE DRUG AND METHOD FOR SCREENING FOR CONFORMATION DISEASE DRUG
WO/2023/036166	PORCINE PLURIPOTENT STEM CELL CULTURE MEDIUM AND USE THEREOF
WO/2024/101323	METHOD FOR PRODUCING CELL POPULATION, AND CELL POPULATION

Inventors:

Nguyen, Tuan Hui
Fourier, Angelique

Application Number:

JP2017543392A

Publication Date:

March 17, 2021

Filing Date:

February 19, 2016

Export Citation:

Click for automatic bibliography generation Help

Assignee:

Anselm (Anstitut Nacional de la Sante et de la Lecherche Medical)
University Dennant

International Classes:

C12N5/071; C07K14/78

Domestic Patent References:

JP2005095138A
JP2011010653A

Foreign References:

WO2014124527A1
WO2014103534A1
WO2014168157A1
WO2011052504A1

Other References:

Molecular Therapy (2011) Vol.19, No.2, pp.400-407
Stem Cell Reports (2013) Vol.1, pp.322-335
Nature Biotechnology (2007) Vol.25, No.6, pp.681-686
「Corning(登録商標)Matrigel(登録商標)基底膜マトリックスよくある質問と回答」、CORNING、pp.1-7、2013年5月[検索日 2019年9月4日]、インターネット
Hepatology (2010) Vol.51, No.1, pp.297-305

Attorney, Agent or Firm:

Ryoichi Takaoka
Nao Oda
Akiyo Iwahori
Kaoru Takahashi

Previous Patent: ポリウレタンベースの転換可能接着剤

Next Patent: 汚れた金属表面を洗浄する方法及びそのような方法に有用な物質