Title:
赤血球老化度の評価方法
Document Type and Number:
Japanese Patent JP6841543
Kind Code:
B2
Abstract:
[Object]It is to provide a method for assessing aging of erythrocytes that can assess aging of erythrocytes with higher accuracy and properly.[Solving means]It is a method for assessing aging of erythrocytes, comprising an erythrocyte suspension-preparing step (S1) for preparing an erythrocyte suspension from a blood sample; a first erythrocyte deformability calculating step (S2) of allowing the erythrocyte suspension to pass through a first filter, separating aged erythrocytes that do not pass through the first filter, and non-aged erythrocytes that pass through the first filter, and to calculate a deformability of erythrocytes; a second erythrocyte deformability calculating step (S3) of allowing the separated non-aged erythrocyte suspension to pass through a second filter having micropores which diameter is smaller than the first filter, separating mild aged erythrocytes that do not pass through the second filter and juvenile erythrocytes that pass through the second filter, and to calculate a deformability of the non-aged erythrocytes; and an assessment step (S4) of assessing aging of erythrocytes, using the deformability of erythrocyte and the deformability of non-aged erythrocyte.
Inventors:
Takehiko Fujino
Sawao Watari
Kaha Tsunemichi
Tetsuji Koyama
Toyoharu Yamashita
Sawao Watari
Kaha Tsunemichi
Tetsuji Koyama
Toyoharu Yamashita
Application Number:
JP2020525543A
Publication Date:
March 10, 2021
Filing Date:
June 10, 2019
Export Citation:
Assignee:
Rheology Functional Food Research Institute Co., Ltd.
International Classes:
G01N33/49
Domestic Patent References:
| JP7051521A | ||||
| JP2015097511A | ||||
| JP2130471A |
Foreign References:
| US20090285892 | ||||
| WO2010137470A1 | ||||
| WO2010047191A1 |
Other References:
上坂伸宏,新しい微小孔フィルター(nickel mesh)を用いて調べた赤血球の変形能,日本バイオレオロジー学会誌,1991年,Vol.5, No.4,pp.23-32
勝沼英宇 ほか,赤血球膜脂肪酸, 膜流動性および変形能と動脈硬化 -動脈硬化の発症進展との因果関係-,動脈硬化,1987年 4月,Vol.15, No.1,pp.43-54
勝沼英宇 ほか,赤血球膜脂肪酸, 膜流動性および変形能と動脈硬化 -動脈硬化の発症進展との因果関係-,動脈硬化,1987年 4月,Vol.15, No.1,pp.43-54
Attorney, Agent or Firm:
High Kazuya Tsu