Title:
キメラ抗原受容体を発現する遺伝子改変Ｔ細胞の調製方法
Document Type and Number:
Japanese Patent JP6857360
Kind Code:
B2
Abstract:
In order to improve the efficiency of gene introduction in CAR therapy employing a transposon method, provided is a method for preparing genetically-modified T cells expressing chimeric antigen receptor, comprising: (1) a step of preparing non-proliferative cells which are obtained by stimulating a group of cells comprising T cells using an anti-CD3 antibody and an anti-CD28 antibody followed by a treatment for causing the cells to lose their proliferation capability; (2) a step of obtaining genetically-modified T cells into which a target antigen-specific chimeric antigen receptor gene has been introduced using a transposon method; (3) a step of mixing the non-proliferative cells prepared by step (1) with the genetically-modified T cells obtained by step (2), and co-culturing the mixed cells while stimulating the mixed cells using an anti-CD3 antibody and anti-CD28 antibody; and (4) a step of collecting the cells after culture. Also, provided is a method for preparing genetically-modified T cells expressing chimeric antigen receptor, comprising: (i) a step of preparing non-proliferative cells holding a viral peptide antigen, which are obtained by stimulating a group of cells comprising T cells using an anti-CD3 antibody and an anti-CD28 antibody followed by culturing in the presence of the viral peptide antigen and a treatment for causing the cells to lose their proliferation capability; (ii) a step of obtaining genetically-modified T cells into which a target antigen-specific chimeric antigen receptor gene has been introduced using a transposon method; (iii) a step of mixing the non-proliferative cells prepared by step (i) with the genetically-modified T cells obtained by step (ii), and co-culturing the mixed cells; and (iv) a step of collecting the cells after culture.
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Inventors:
Nishio Nobuhiro
Nakazawa Yozo
Miyuki Tanaka
Daisuke Morita
Takahashi Yoshiyuki
Nakazawa Yozo
Miyuki Tanaka
Daisuke Morita
Takahashi Yoshiyuki
Application Number:
JP2017544248A
Publication Date:
April 14, 2021
Filing Date:
October 07, 2016
Export Citation:
Assignee:
Tokai National University Organization
Shinshu University
Shinshu University
International Classes:
C12N15/85; A61K35/17; A61P35/00; C12N5/0783; C12N5/10
Other References:
Cytotherapy,2014年,Vol.16,pp.1257-1269
信州医誌,2013年,Vol.61, No.4,pp.197-203
PLoS ONE,2015年 6月,Vol.10, No.6, e0128151,pp.1-19
信州医誌,2013年,Vol.61, No.4,pp.197-203
PLoS ONE,2015年 6月,Vol.10, No.6, e0128151,pp.1-19
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office
Mikiharu Hagino
Mikiharu Hagino