Title:
生体高分子３次元構造再構成の検証システム
Document Type and Number:
Japanese Patent JP6883235
Kind Code:
B2
Abstract:
To provide a system for verifying an analysis result using biopolymer single particle analysis method with physically sufficient validity.SOLUTION: The system for verifying a biopolymer three-dimensional structure reconfiguration comprises: means (1-5) for calculating a TEM simulated image (1-16) captured in an arbitrary projection direction on the basis of a three-dimensional molecular structure (1-15) estimated from a biopolymer TEM image (1-10) obtained by a cryogenic transmission electron microscope (1-9); means (1-6) for calculating a degree of coincidence between the biopolymer TEM image (1-10) and the TEM simulated image (1-16); means (1-7) for estimating a three-dimensional potential distribution simulated image (1-17) from the TEM simulated image (1-16), as a three-dimensional reconfiguration structure; and means (1-8) for calculating a degree of coincidence between a three-dimensional potential distribution image (1-13), which is estimated from the biopolymer TEM image (1-10) as a three-dimensional reconfiguration structure, and the three-dimensional potential distribution simulated image (1-17).SELECTED DRAWING: Figure 1
Inventors:
Takao Shinkawa
Fumio Hosokawa
Masataka Ohashi
High Seizo Hashi
Kazushige Mori
Fumio Hosokawa
Masataka Ohashi
High Seizo Hashi
Kazushige Mori
Application Number:
JP2019125185A
Publication Date:
June 09, 2021
Filing Date:
July 04, 2019
Export Citation:
Assignee:
BioNet Laboratory, Inc.
International Classes:
H01J37/153; G01N23/04; H01J37/21; H01J37/22; H01J37/26
Domestic Patent References:
| JP2010117175A | ||||
| JP2005250721A |
Foreign References:
| WO2002075658A1 | ||||
| US20040114806 | ||||
| WO2010083851A1 |
Other References:
上野 豊 他,電子顕微鏡によるタンパク質立体構造の単粒子解析,日本物理学会誌,2002年,Vol.57 No.8,pp568-574,DOI https://doi.org/10.11316/butsuri1946.57.568,URL,https://www.jstage.jst.go.jp/article/butsuri1946/57/8/57_8_568/_pdf/-char/ja
宮崎 直幸 他,創薬等ライフサイエンス用クライオ電子顕微鏡単粒子解析支援システム,顕微鏡,2018年,Vol.53 No.1,pp8-12,DOI https://doi.org/10.11410/kenbikyo.53.1_8,URL,https://www.jstage.jst.go.jp/article/kenbikyo/53/1/53_8/_pdf/-char/ja
宮崎 直幸 他,創薬等ライフサイエンス用クライオ電子顕微鏡単粒子解析支援システム,顕微鏡,2018年,Vol.53 No.1,pp8-12,DOI https://doi.org/10.11410/kenbikyo.53.1_8,URL,https://www.jstage.jst.go.jp/article/kenbikyo/53/1/53_8/_pdf/-char/ja
Attorney, Agent or Firm:
Yoshinaga Takahiro