核酸含有組成物の作製および精製のためのプロセス - The United States of America, As Represented by The Secretary, Department of Health and Human Services

Title:

核酸含有組成物の作製および精製のためのプロセス

Document Type and Number:

Japanese Patent JP6886410

Kind Code:

B2

Abstract:

Described are improved processes for production and purification of nucleic acid-containing compositions, such as non-naturally occurring viruses, for example, recombinant polioviruses that can be employed as oncolytic agents. Some of the described improved processes relate to improved processes for producing viral DNA template. Also described are improved processes for chromatography purification of nucleic acid-containing compositions, in which the nucleic acid is quantified in chromatography fractions using a rapid detection method of the one or more nucleic acid sequences of the nucleic acid-containing composition, such as detection by real time RT-qPCR. In addition, improved processes for production and purification of oncolytic poliovirus, such as PVS-RIPO, are described. Compositions generated using these methods are also provided.

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Inventors:

Broad, Trevor Lane
Chaban, Simir Hussein
Che, Yuetin
Jue, jun way shaun
George Mitra

Application Number:

JP2017563547A

Publication Date:

June 16, 2021

Filing Date:

June 10, 2016

Export Citation:

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Assignee:

The United States of America, As Represented by The Secretary, Department of Health and Human Services

International Classes:

C12N7/02; B01D15/34; B01D15/36; C12N15/10; C12N15/43; C12N15/63; C12Q1/6851; G01N30/02

Domestic Patent References:

JP58500589A
JP2006528886A
JP5503634A

Foreign References:

CN1616095A

Other References:

Proc. Natl. Acad. Sci. U.S.A. (1996) vol.93, no.6, p.2370-2375
村松正實編, 分子細胞生物学辞典, 2002年2月, 株式会社東京化学同人, p.486
TaKaRaバイオ総合カタログ2002-2003, p.R-21
Mol. Ther. (2008) vol.16, no.11, p.1865-1872

Attorney, Agent or Firm:

Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto

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