Title:
生存微生物の迅速な計数のためのスペクトル強度比（ＳＩＲ）分析
Document Type and Number:
Japanese Patent JP6991274
Kind Code:
B2
Abstract:
The present invention relates to a method for differentiating between live and inactivated bacteria in a whole sample comprising the steps of (i) staining the whole sample with a single membrane-associated dye, (ii) illuminating the stained whole sample with an incident light having an excitation wavelength, (iii) measuring an emission intensity at a range of emitted wavelengths, (iv) creating, for the whole sample, a first emission fluorescence spectra from the plurality of emitted wavelengths, and (v) comparing the first emission fluorescence spectra with an emission fluorescence spectra generated from a sample comprising live bacteria only to determine a spectral shift. Further, the invention also relates to a method for differentiating the relative amount of live and inactivated bacteria in a treated sample comprising staining the whole sample with a single membrane-associated dye; illuminating the whole sample with an incident light at λ excitation; measuring, for the whole sample, (i) the intensity I1 of emitted light at wavelength λ1; and (ii) the intensity I2 of emitted light at wavelength λ2; calculating a ratio I2/I1; and determining the relative amounts of live and inactivated bacteria based on whether the calculated I2/I1 is larger or smaller than a predetermined threshold.
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Inventors:
Zahavi, Eran
Application Number:
JP2020097137A
Publication Date:
January 12, 2022
Filing Date:
June 03, 2020
Export Citation:
Assignee:
TACOUNT EXACT LTD.
International Classes:
C12Q1/06; C12Q1/22; G01N33/48; G01N33/483
Domestic Patent References:
| JP2018508190A | ||||
| JP2011527562A | ||||
| JP2013538567A | ||||
| JP2002291499A | ||||
| JP2002168870A | ||||
| JP7196930A |
Foreign References:
| WO2011086990A1 |
Other References:
Journal of Microbiological Methods, 2006, Vol.65, p.335-345
Peptides, 2009, Vol.30, p.2144-2149
Peptides, 2009, Vol.30, p.2144-2149
Attorney, Agent or Firm:
Akio Miyazaki
Masaaki Ogata
Masaaki Ogata
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