Title:
グリコシル化ペプチドの検出及び定量
Document Type and Number:
Japanese Patent JP7412407
Kind Code:
B2
Abstract:
A method of purification and/or separation of glycopeptides and quantitation of same. The method includes contacting a sample comprising glycopeptides to a hydrophilic enrichment substrate under conditions that permit the glycopeptides to bind to the hydrophilic enrichment substrate. The glycopeptides are eluted from the hydrophilic enrichment substrate with an ammonium formate and acetonitrile (ACN) in water solution to create an enriched glycopeptide sample, which may be subjected to analysis to identify specific glycopeptides.
Inventors:
Rosenberg Abraham Zed.
Shen Biao
Shen Biao
Application Number:
JP2021500860A
Publication Date:
January 12, 2024
Filing Date:
July 12, 2019
Export Citation:
Assignee:
REGENERON PHARMACEUTICALS, INC.
International Classes:
G01N30/88; B01J20/288; C07K1/16; G01N30/00; G01N30/06; G01N30/26; G01N30/34; G01N30/72
Domestic Patent References:
| JP2015121539A |
Foreign References:
| WO2017037829A1 | ||||
| WO2017189357A2 | ||||
| US20090294362 |
Other References:
Kondo, A. et al.,Characterization of sialyated and fucosylated glycopeptides of beta2-glycoprotein l by a combination of HILIC and MALDI MS/MS,J. Sep. Sci.,2010年,vol.33,pp.891-902
Attorney, Agent or Firm:
Hatsushi Shimizu
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto