Title:
【発明の名称】粒状固定化リパーゼ調製品、その製法およびその使用
Document Type and Number:
Japanese Patent JPH04501664
Kind Code:
A
Abstract:
PCT No. PCT/DK89/00270 Sec. 371 Date Apr. 24, 1991 Sec. 102(e) Date Apr. 24, 1991 PCT Filed Nov. 15, 1989 PCT Pub. No. WO90/05778 PCT Pub. Date May 31, 1990.A particulate immobilized microbial lipase is prepared by adsorption of lipase to macroporous inorganic carrier particles having specific characteristics. The carrier particles contain at least 65% silica or silicates, an excess of 90% particles having a size between 100 and 1000 mu m and an excess of 80% pores exhibiting a diameter of between 12 and 45 times the diameter of a lipase molecule. The lipase is adsorbed to the carrier particles by contacting the particles with an aqueous solution of lipase and drying the particles to a water content between 1 and 20%. The lipase is preferably a thermostable lipase from a Humicola species, Candida antarctica or Rhizomucor miehei. The particulate immobilized lipase is cheap to produce, exhibits a high specific activity, and can be used for interesterification of fats, hydrolysis of fats or synthesis of fatty acid esters.
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Inventors:
Sven, Pedersen
Hansen, Thomas Taye
Hansen, Thomas Taye
Application Number:
JP50013989A
Publication Date:
March 26, 1992
Filing Date:
November 15, 1989
Export Citation:
Assignee:
NOVO NORDISK AKTIE SELSXAB
International Classes:
C12N9/20; C12N11/14; (IPC1-7): C12N11/14
Attorney, Agent or Firm:
Aoki Akira (3 outside)