Title:
【発明の名称】改変されたNα―アセチルトランスフェラーゼ活性を有するサッカロミセス・セレビシエ株の単離
Document Type and Number:
Japanese Patent JPH05501803
Kind Code:
A
Abstract:
A mutation defining a gene for the N< alpha >-acetyltransferase of yeast has been identified and cloned. Mutations in this gene have been constructed and used to produce cells which are substantially incapable of catalyzing N< alpha >-acetylation of protein. Such cells are valuable tools for determining the amino acid sequence of uncharacterized proteins. Such cells are also valuable tools for expressing a recombinant protein lacking an acetyl group at its alpha -amino group.
Inventors:
Smith, john a
Lee, Fang Jen S
Lee, Fang Jen S
Application Number:
JP51557390A
Publication Date:
April 08, 1993
Filing Date:
October 15, 1990
Export Citation:
Assignee:
The General Hospital Corporation
International Classes:
C12N1/21; C12N9/10; C12N15/54; C12N15/82; C12P21/00; C12Q1/02; C12N1/19; C12Q1/48; G01N33/68; C12R1/865; (IPC1-7): C12N1/19; C12N9/10; C12N15/54; C12P21/00; C12Q1/02; C12Q1/48
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)