Title:
【発明の名称】分泌大腸菌突然変異体からのヒトインターロイキン―4の精製
Document Type and Number:
Japanese Patent JPH05505934
Kind Code:
A
Abstract:
Active recombinant human IL-4 is purified from a crude fermentation broth of a secreted E. coli mutant by subjecting said solution to selective chromatogrphy on a zinc-chelating agarose column at pH 7.2 in a buffer containing a high concentration of sodium chloride, i.e., about 1M sodium chloride, washing first with a phosphate buffer at pH 7.2 containing 1.0M sodium chloride then with a buffer containing 10% glycerol and eluting at pH 5.0. The resulting purified active recombinant human IL-4 solution is further treated by cation exchange chromatography, concentration by diafiltration up to 20 mg/mL and size exclusion gel chromatography using a citrate buffer at pH 4.5.
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Inventors:
Tan, John Chutai
Nerve, David
Naga Buchamp, Thiel
Nerve, David
Naga Buchamp, Thiel
Application Number:
JP50352891A
Publication Date:
September 02, 1993
Filing Date:
January 04, 1991
Export Citation:
Assignee:
SCHERING CORPORATION
International Classes:
C07K1/16; C07K1/22; C07K14/52; C07K14/54; C12N15/09; C12P21/00; C07K14/435; C12P21/02; A61K38/00; C12R1/19; (IPC1-7): C12P21/02; C07K3/20; C07K15/06; C12P21/00
Domestic Patent References:
| JPH04506299A | 1992-11-05 |
Attorney, Agent or Firm:
Kyozo Yuasa (6 people outside)