Title:
【発明の名称】ホスホロチオエートオリゴヌクレオチドのクロマトグラフィー分離
Document Type and Number:
Japanese Patent JPH06503356
Kind Code:
A
Abstract:
PCT No. PCT/US92/08835 Sec. 371 Date May 10, 1993 Sec. 102(e) Date May 10, 1993 PCT Filed Oct. 15, 1992.A method is provided for the preparation and/or analysis of synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on an anion exchange HPLC columns using concentration gradients of novel "soft base" anionic eluents, such as bromide, thiocyanate, and the like.
Inventors:
Belgod, B. John
Application Number:
JP50560893A
Publication Date:
April 14, 1994
Filing Date:
October 15, 1992
Export Citation:
Assignee:
Lynx Therapeutics, Incorporated
International Classes:
B01D15/08; C07H1/06; C07H21/00; C12N15/10; B01D15/04; (IPC1-7): C07H1/06; B01D15/04; B01D15/08; C07H21/00
Attorney, Agent or Firm:
Eiko Funahashi