Title:
【発明の名称】ランダムコドンを有するオリゴヌクレオチドを合成する方法
Document Type and Number:
Japanese Patent JPH06503809
Kind Code:
A
Abstract:
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.
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Inventors:
Husse, William Dee.
Application Number:
JP51511591A
Publication Date:
April 28, 1994
Filing Date:
August 20, 1991
Export Citation:
Assignee:
Igus®, Incorporated
International Classes:
C07H21/00; C07H21/04; C12N15/00; C12Q1/68; (IPC1-7): C07H21/04; C07H21/04
Attorney, Agent or Firm:
Shusaku Yamamoto