Title:
【発明の名称】電気泳動法におけるまたは関する改良
Document Type and Number:
Japanese Patent JPH06505473
Kind Code:
A
Abstract:
PCT No. PCT/GB92/00249 Sec. 371 Date Jul. 27, 1993 Sec. 102(e) Date Jul. 27, 1993 PCT Filed Feb. 12, 1992 PCT Pub. No. WO92/14747 PCT Pub. Date Sep. 3, 1992.A method of separating or distinguishing proteins by isoelectric focusing (IEF), comprises fluorescently labelling proteins by reacting monobromobimane or monochlorobimane with free sylphydryl groups of proteins, and subjecting the labelled proteins to isoelectric focusing. The IEF may be followed by a second electrophoretic separation step, resulting in 2-D electrophoresis of flourescently pre-labelled proteins in a way that has not hitherto been possible.
Inventors:
Peter Jackson
Application Number:
JP50417292A
Publication Date:
June 23, 1994
Filing Date:
February 12, 1992
Export Citation:
Assignee:
Astrocom Limited
International Classes:
C07K1/00; C07K1/107; C07K1/113; C07K1/24; C07K1/26; C07K1/28; G01N27/447; (IPC1-7): C07K3/08; C07K3/14; G01N27/447
Attorney, Agent or Firm:
Fukami Hisaro (3 outside)