【発明の名称】グラム陽性細菌中での外層膜タンパク質の生産及び保護的エピトープの回復 - The Finish National Public Health Institute

Title:

【発明の名称】グラム陽性細菌中での外層膜タンパク質の生産及び保護的エピトープの回復

Document Type and Number:

Japanese Patent JPH06511140

Kind Code:

A

Abstract:

The invention provides a method for producing cloned outer membrane (OM) protein from pathogenic gram-negative bacteria. The invention also provides a method for renaturing the cloned outer membrane protein thus produced so the cloned OM protein regains immunologically active epitopes which are capable of eliciting production of antibodies, in mammals and other animals, that are bactericidal and can provide protection against infection by the pathogenic gram-negative bacteria. According to the method, DNA encoding outer membrane protein from gram-negative bacteria, known to be pathogenic in humans and animals, is expressed in a gram-positive bacterial host. The recombinant or cloned OM protein thus produced is then renatured so as to regain biologically or immunologically active epitopes which are capable of eliciting production of antibodies in animals and humans; the antibodies are bactericidal and protect the animals and humans from infection by the pathogenic gram-negative bacteria from which the gene encoding the cloned OMP's was derived. The method of the invention is exemplified in part by the production of cloned and renatured class 1 outer membrane protein from Neisseria meningitidis, class 3 OM protein of Neisseria meningitidis and the OM protein OmpA of Escherichia coli.

More Like This:

JP2021518414	Compositions Containing Bacterial Strains
JP2022540197	Ehrlichia vaccines and immunogenic compositions
WO/1999/058146	ATTENUATED MUTANTS OF $i(SALMONELLA) WHICH CONSTITUTIVELY EXPRESS THE Vi ANTIGEN

Inventors:

Servous Mathie
Butcher Salah
Naminen-Kariokoski Marjatta
Luneburg-Nayman Kate
Mattie linen susanna
Warstrom Eva
Idampan-Heikkiiraona
Puohini Emi Litovarena

Application Number:

JP51165891A

Publication Date:

December 15, 1994

Filing Date:

July 05, 1991

Export Citation:

Click for automatic bibliography generation Help

Assignee:

The Finish National Public Health Institute

International Classes:

A61K39/02; A61K39/095; A61K39/10; A61K39/102; A61K39/108; A61P31/04; C07K14/005; C07K14/195; C07K14/22; C07K14/245; C07K14/41; C07K14/705; C12N15/09; C12N15/75; C12P21/02; A61K38/00; A61K39/00; C12R1/36; C12R1/91; (IPC1-7): C12P21/02; A61K39/02; A61K39/095; A61K39/10; A61K39/102; A61K39/108; C07K15/04; C12N15/31

Attorney, Agent or Firm:

Eijiro Tanigawa

Previous Patent: 不均一なガス流によるレチクル冷却

Next Patent: JPH06511141