【発明の名称】第IX因子タンパク質の製造方法

Japanese Patent JPH082300

B2

The blood-clotting protein, factor IX, is synthesised in the body in liver cells, where it undergoes three distincttypes of post-translational modification before it is secreted into the bloodstream as a 415 amino acid long protein. It is therefore a difficult protein to produce by recombinant DNA technology in a highly biologically active form. Nevertheless, such a result has been achieved by the present invention in which typically factor IX cDNA in a plasmid is linearised and inserted into an expression vector having a promotor sequence of SV40 early gene, an SV40 polyadenylation sequence, the TK/NEO selectable marker and an ampicillin resistance gene. Mammalian cells such as from a dog kidney or rat liver are transfected by the calcium phosphate precipitation method. High levels of factor IX in a fully or near-fully biologically active form, useful as a plasma-free preparation for treatment of patients suffering from Christmas Disease (haemophilia B), are obtainable without recourse to poxvirus vectors which would contaminate the protein.

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JP6220794	A structure and a method for synthetic 2 direction vegetable promotor UBI1
JP4416494	Probe sets and methods for identifying HLA-DQ alleles

Donald Stewart Anson
Jioji Gou Brownlee
Ian martyne jyons

JP5674586A

January 17, 1996

March 14, 1986

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British Technology Group Limited

C12N15/09; A61K35/12; A61K38/16; A61K38/43; C07K1/22; C07K14/00; C07K14/745; C07K16/40; C12N9/64; C12N15/00; C12N15/57; A61K38/00; C12R1/91; (IPC1-7): C12N9/64

【文献】英国特許2125409(GB,A)

Yoshio Kawaguchi (2 outside)

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