Title:
【発明の名称】妊娠哺乳類の羊膜破壊の検出法
Document Type and Number:
Japanese Patent JPH08508015
Kind Code:
A
Abstract:
PCT No. PCT/AU94/00144 Sec. 371 Date Sep. 7, 1995 Sec. 102(e) Date Sep. 7, 1995 PCT Filed Mar. 23, 1994 PCT Pub. No. WO94/21687 PCT Pub. Date Sep. 29, 1994The present invention relates to the method of detecting rupture of the amniotic membranes in pregnant mammals including humans using an immunoassay and reagents useful in such an assay. The method describes how to prepare a suitable protein antigen from amniotic fluid, gives criteria for the selection of this protein from the mixture of proteins present in amniotic fluid using the techniques of protein purification, gives criteria for assessing a sufficient degree of antigen purity for raising antibodies to the antigen and shows how the resultant antibodies can be used in immunoassays to detect the presence of amniotic fluid in the vagina and consequently to detect rupture of the amniotic membranes. The method also relates to the detection of amniotic fluid in other situations.
Inventors:
Boroteriak, Victor
Cowley, David Michael
Cowley, David Michael
Application Number:
JP52043194A
Publication Date:
August 27, 1996
Filing Date:
March 23, 1994
Export Citation:
Assignee:
Boroteriak, Victor
Cowley, David Michael
Cowley, David Michael
International Classes:
G01N33/53; C07K1/18; C07K14/47; C07K16/18; C12N5/10; C12N15/02; C12P21/08; G01N33/577; C12R1/91; (IPC1-7): C07K14/47; C07K1/18; C07K16/18; C12N5/10; C12N15/02; C12P21/08; G01N33/53; G01N33/577
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)