Title:
【発明の名称】多酵素及びCMP-シアル酸再生系を使用するオリゴ糖のワンポット合成
Document Type and Number:
Japanese Patent JPH08509619
Kind Code:
A
Abstract:
A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a beta -galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an alpha -sialyltransferase. The value of Km/Vmax for the formed galactosyl glycoside as a substrate for the alpha -sialyltransferase is less than one-third the Km/Vmax value for the galactose-containing substrate for that alpha -sialyltransferase.
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Inventors:
Wonchi Huey
Gaei Federico Sea A
Gaei Federico Sea A
Application Number:
JP52466794A
Publication Date:
October 15, 1996
Filing Date:
May 04, 1994
Export Citation:
Assignee:
The Scripps Research Institute
International Classes:
C12N9/10; C12N9/38; C12P19/14; C12P19/18; C12P19/26; (IPC1-7): C12P19/14; C12P19/18
Attorney, Agent or Firm:
Minoru Nakamura (7 outside)