Title:
【発明の名称】新規セファロスポリンCアシラーゼ
Document Type and Number:
Japanese Patent JPH09505732
Kind Code:
A
Abstract:
PCT No. PCT/JP94/01799 Sec. 371 Date May 1, 1996 Sec. 102(e) Date May 1, 1996 PCT Filed Oct. 26, 1994 PCT Pub. No. WO95/12680 PCT Pub. Date May 11, 1995A mutant CC acylase wherein at least one amino acid at the Ala49, Met164, Ser166, Met174, Glu358, Met465, Met506, or Met750 position of the amino acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.
Inventors:
Niwa Mineo
Yoshimasa Saito
Takamura Fujimura
Yoshinori Ishii
Noguchi Yuji
Yoshimasa Saito
Takamura Fujimura
Yoshinori Ishii
Noguchi Yuji
Application Number:
JP51311995A
Publication Date:
June 10, 1997
Filing Date:
October 26, 1994
Export Citation:
Assignee:
Fujisawa Pharmaceutical Co., Ltd.
International Classes:
C12N1/21; C12N9/14; C12N9/80; C12N15/55; C12P35/02; C12N15/09; C12R1/19; (IPC1-7): C12N15/09; C12P35/02; C12N9/14; C12N1/21
Attorney, Agent or Firm:
Takashima Hajime