Title:
【発明の名称】普遍的なドナー細胞及びキメラ性哺乳類宿主のための相同組換え
Document Type and Number:
Japanese Patent JPH09507753
Kind Code:
A
Abstract:
Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, each of the beta 2- microglobulin gene and the IFN- gamma R gene is inactivated for reducing or eliminating the expression of functional MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.
Inventors:
Kucharapati, Large
Coller, Beverly H.
Smithy, Oliver
Dubridge, Robert Bee.
Gary, Greenberg
Capon, Daniel Jay.
Williams, Stephen Earl.
Rhodes Arbons de Rafael, Maria
Coller, Beverly H.
Smithy, Oliver
Dubridge, Robert Bee.
Gary, Greenberg
Capon, Daniel Jay.
Williams, Stephen Earl.
Rhodes Arbons de Rafael, Maria
Application Number:
JP51816195A
Publication Date:
August 12, 1997
Filing Date:
December 28, 1994
Export Citation:
Assignee:
Cell Genesis, Incorporated
International Classes:
A01K67/027; A61K35/12; A61K38/21; A61K45/00; C07H21/04; C07K14/74; C12N5/02; C12N5/10; C12N15/00; C12N15/09; C12N15/85; C12Q1/68; G01N33/53; (IPC1-7): C12N15/09; A61K35/12; A61K45/00; C07H21/04; C12N5/10
Attorney, Agent or Firm:
Takashi Ishida (3 others)