Title:
中規模ポリヌクレオチド増幅装置
Document Type and Number:
Japanese Patent JPH09511407
Kind Code:
A
Abstract:
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction chamber, having at least one cross-sectional dimension of about 0.1 to 1000 mu m. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation. The amplification reaction chamber also may be provided with a composition which diminishes inhibition of the amplification reaction by material comprising a wall of the reaction chamber, when such treatment is required.
Inventors:
クリッカ、ラリー・ジェイ
ワイルディング、ピーター
ワイルディング、ピーター
Application Number:
JP51629496A
Publication Date:
November 18, 1997
Filing Date:
November 13, 1995
Export Citation:
Assignee:
UNIV PENNSYLVANIA (US)
International Classes:
G01N33/48; B01D35/00; B01D35/02; B01D61/18; B01D67/00; B01D71/02; B01J19/00; B01L3/00; B01L99/00; C12M1/00; C12M1/34; C12N15/09; C12Q1/68; G01N1/28; G01N37/00; (IPC1-7): C12M1/00; C12Q1/68
Attorney, Agent or Firm:
青山 葆 （外１名）